Fig. 3.

Effect of 2,3-dehydrosilydianin (DHSD) on ARE-driven gene expression in AREc32 reporter cells. Cells were treated for 24 h with 0.1% DMSO (control), 2.5 μM sulforaphane (SFN; positive control) or with 12.5–50 μM DHSD. After treatment, luciferase reporter activity was determined luminometrically and normalized to protein content. Data are means ± standard deviation of three experiments. *p < 0.05; **p < 0.01, significantly increased versus control.