Table 3. Real time qPCR verification of RNA sequencing.
Results presented as fold change (FC) for eight genes. The (n.a.*) refers to not differentially expressed, while the fold change was not available for this gene in this group.
| Gene ID | FC RNA-seq | FC RT qPCR | |||||
|---|---|---|---|---|---|---|---|
| Dose rate (mGy/h) | 0.54 | 5.4 | 10.9 | 0.54 | 5.4 | 10.9 | |
| pfkfb3 | 1.8 | 1.3 | 1.5 | 1.8 | 1.4 | 0.9 | |
| crabp2b | 1.9 | 2.1 | 2.0 | 2.9 | 2.3 | 2.0 | |
| vox | 1.5 | 1 | 1.5 | 1.6 | 2.2 | 0.7 | |
| ppp1r15a | 1.6 | 0.8 | 1.5 | 1.8 | 1.3 | 0.6 | |
| shisa2 | 1.3 | 1 | 1.3 | 1.5 | 1.9 | 0.7 | |
| sox2 | n.a.* | 3.3 | 2.2 | 3.0 | 1.6 | 0.8 | |
| tfa | n.a.* | 93.2 | 3.2 | 1.2 | 40.7 | 20.7 | |
| eef2b | 1.1 | 1.3 | 1.2 | 0.6 | 1.6 | 1.4 | |