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. 2017 Jun 7;13(6):e1006347. doi: 10.1371/journal.ppat.1006347

Fig 2. ERRα knockdown increases the expression of multiple antiviral genes.

Fig 2

(A-B) Microarray analysis of shCtrl and shERRα-2 293T cells following VSV infection for 12 h. (A) Enrichment of KEGG pathways by FunNet analysis for genes upregulated or downregulated in shERRα-2-VSV cells relative to their expression in shCtrl-VSV cells (adjusted to FC ≥ 2) in at least one comparison. (B) Heatmap of upregulated IFN-I responsive genes. The color represents normalized expression of genes in shERRα-2 cells relative to their expression in shCtrl cells. (C-E) qPCR analysis of ESRRA, IFNB1, IFIT1, IFIT2, IFIT3, IFIH1 and LILRB2 mRNA expression in shCtrl and shERRα-2 293T cells infected with VSV for the indicated times. The data were normalized to the expression of the β-Actin reference gene. Cell-based studies were performed independently at least three times with comparable results. The data are presented as the means ± SEM.