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. 2017 Jun 7;13(6):e1006347. doi: 10.1371/journal.ppat.1006347

Fig 7. ERRα is stabilized by viral infection.

Fig 7

(A-E) Immunoblotting analysis of ERRα protein expression in 293T (A), BMDMs (B), THP-1(C), MEFs (D), and HeLa (E) infected with VSV (MOI = 1.0) for the indicated times. α-Tubulin was used as the equal loading control. (F-G) Immunoblotting analysis of ERRα protein expression in THP-1 cells infected with HSV (F) or incubated with 100 ng/ml LPS (G) for the indicated times. α-Tubulin was used as the equal loading control. (H) Immunoblotting analysis of ERRα protein expression from tissues of VSV-infected C57BL/6 mice collected at the indicated time points (n = 3 per group). α-Tubulin was used as the equal loading control. (I) Immunoblotting analysis of fractionated 293T cells infected with VSV for the indicated times. Cyt, cytosolic; Nuc, nuclear. The purity of the fractions was assessed by blotting for Lamin A (nuclear protein) and α-Tubulin (cytosolic protein). (J) qRT-PCR analysis of ERRα mRNA expression in 293T cells infected with VSV (MOI = 1.0) for the indicated times. The data were normalized to the expression of the β-actin reference gene. Cell-based studies were performed independently at least three times with comparable results. The data are presented as the means ± SEM.