Figure 3. Blood stage infection alters differentiation of CSP-specific B cells.

(A, left) Splenocytes were isolated from Pyfabb/f⁻ or PyWT infected mice harvested on day 6, 14, 30 and 90 and analyzed by flow cytometry for CSP⁺CD38^low CD138^hi plasmablasts. (A, right) Absolute number of CSP-specific CD38^low CD138^hi cells. (B, left) Splenocytes from Pyfabb/f⁻ or PyWT infected mice were analyzed for CSP⁺CD38^low CD138⁻ GL7^hi germinal center B cells, on days 6, 14, 30 and 90 by flow cytometry. (B, right) Absolute numbers of CSP⁺CD38^low CD138⁻ GL7^hi cells. Results are representative of 2–3 independent experiments with 3–6 mice in each. Error bars represent SD. See also figure S3.