Response of heart rate and respiratory rate to surgical stimuli. Surgical stimuli in chronological order: skin incision (SI), abdominal wall incision (AI), ovary manipulation (O), abdominal wall suture (AS), skin stapling (ST), and injection of analgesic agent (I). (a) Mean ± SD (n = 7) heart rate deviation in beats per minutes (Δbpm) compared to baseline values obtained 10 s prior to applying stimulus. (b) Mean ± SD (n = 7) respiratory rate deviation in breaths per minute (Δ brpm). Asterisks indicate significance (P ≤ 0.05) compared to baseline obtained 10 s prior to applying stimulus by counting movements of the thorax wall