Figure 2.

Pancreatic islets from high fat-fed mice show altered insulin secretion that was not observed after treatment with butyrate. Batches of isolated islets from mice of all experimental groups were exposed to 2.8 (a) or 16.7 mM glucose (b) for 60 min. Compared to controls (C), islets from HF-fed mice (HF) showed increased basal insulin secretion (expressed as ng.mL⁻¹.islet⁻¹) in the presence of 2.8 mM glucose (G 2.8) but a comparable insulin release when stimulated by 16.7 mM glucose (G 16.7) (b). This increased basal secretion was not observed in HF-fed mice receiving diet supplementation with butyrate (HFB) as compared to its control (CB)(a); but a tendency of an increase in stimulated insulin release (b) was verified in mice with butyrate diet supplementation (CB and HFD groups) as compared to those not receiving this SCFA (C and HF groups). Bars represent means + SE of four independent experiments (10–14 batches of five islets isolated from four mice per group). *P < 0.05 compared to C group