Figure 3.
Representative western blots of explants treated for 18 hours with TGFβ with or without UO126 pre-treatment prior to TGFβ. Phosphorylated-Smad2/3, total-Smad2/3, total-ERK1/2 and GAPDH are labeled (A). p-Smad2/3, t-Smad2/3 and t-ERK1/2 were normalized against GAPDH (B; error bars represent S.E.M.). ANOVA with Sidak’s post-hoc test was used to analyze data (*p<0.05; **p<0.01; ****p<0.0001).