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. Author manuscript; available in PMC: 2017 Jun 19.
Published in final edited form as: Methods Mol Biol. 2016;1441:43–56. doi: 10.1007/978-1-4939-3684-7_4

Table 3. Master Mix for FCGR3A SNP and FCGR2C SNP.

Multiply the number of reactions (# of unknowns= samples * # replicates/sample + # of no template samples + # control samples) * 1.1. Refer to Note 13 for each component by the reaction volume for 1 reaction (RXN).

FCGR3A FCGR2C
Volume (μL) 1 Reaction Final Concentration Volume (μL) 1 Reaction Final Concentration
2X Genotyping Master Mix (ABI) 2.5 1X 2X Express Genotyping Master Mix (Invitrogen) 2.5 1X
RH FCGR3A Forward Primer (10uM) 0.15 0.3uM FCGR2C Forward Primer (10uM) 0.15 0.3uM
FCGR3A Reverse Primer (10uM) 0.15 0.3uM RH FCGR2C Reverse Primer (10uM) 0.15 0.3uM
FCGR3A-G Probe (10uM) 0.125 0.25uM FCGR2C-C Probe (10uM) 0.125 0.25uM
FCGR3A-T-Probe (10uM) 0.125 0.25uM FCGR2C-T-Probe (10uM) 0.125 0.25uM
RH Enzyme in Buffer (25U/uL) 0.2 1U/uL RH Enzyme in Buffer (25U/uL) 0.2 1U/uL
DNA (5ng/uL) 1 N/A DNA (5ng/uL) 1 N/A
Sterile Water 0.75 N/A Sterile Water 0.75 N/A