Author response image 7. Analysis of tissue folding during placode formation in FGF3 overexpressing embryos.
FGF3 expression was induced with a heat shock by incubating the 11 hpf embryos for 30 min at 39 degrees. For this purpose, the Tg(hsp70:fgf3) line was used. The embryos were injected at 1 cell stage with memb-GFP. (A) Note that the anterior region folds before the posterior one, which remains incompletely folded at late stages (20hf). Arrowhead: a deformation of the lumen can be observed in the posterior region of the vesicle, which is found only anteriorly in wild type embryos and associated to the unfolded tissue. (B) Detail of the posterior unfolded tissue.