Video 12. Ablation of pioneer cells before ingression affects neurog1 expression in the neurogenic domain at later stages.
3D reconstruction: DsRedE signal in the neurogenic domain (red) of otic vesicles at 21 hpf corresponding to the previously ablated and contralateral non-ablated sides of the same embryo. A single plane of the memb-GFP signal from each vesicle is shown for better 3D orientation (green). The DsRedE fluorescence coming from cells outside the otic vesicle was removed with FIJI to improve the visualisation of the phenotype inside the vesicle. z-stack: sequence of coronal planes from dorsal to ventral of neurog1 expression in the otic vesicle at 21 hpf in ablated and contralateral non-ablated sides of the embryo. The DsRedE expression levels can be visualised in single cells (quantifications of specification phenotypes were performed on this type of z-stacks).