Figure 5. Chemotaxis to live bacteria in the presence of competing chemoattractants.
(A) Time-course quantification of WT Dictyostelium migration to 5 × 107 Bacillus subtilis or to buffer controls in the presence of competing levels of 5 μM folate or 5 μM cAMP in the top well, as indicated. Relative chemotaxis is normalized to WT Dictyostelium at 1.5 hr without any competitor. Standard deviations are shown based upon three replicates. (B) Time-course quantification of WT Dictyostelium migration to 5 × 107 Klebsiella planticola or to buffer controls in the presence of competing levels of 5 μM folate or 5 μM cAMP in the top well, as indicated. Relative chemotaxis is normalized to WT Dictyostelium at 1.5 hr without any competitor. Standard deviations are shown based upon three replicates.