Skip to main content
. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Exp Cell Res. 2017 Jan 26;352(1):20–33. doi: 10.1016/j.yexcr.2017.01.013

Figure 2.

Figure 2

Lactate oxidation metabolite (LOM)-mediated growth of MDA-MB-231 cells – Modulation of MDA-MB-231 cell growth in the absence or presence of 2.5 mM glucose (Gluc) by 2.5 mM of (A) α-alanine (α-Ala), β-alanine (β-Ala), GABA, acetate (Acet) or (B) by 2.5 mM pyruvate (sodium pyruvate). Compared to control cells exposed to culture medium without glucose and added nutrients (Dep), neither GABA, alanine or acetate produced a significant growth benefit for MDA-MB-231 cells; While α-alanine appears to increase cell growth in the presence of 2.5 mM glucose, GABA, β-alanine, and acetate abrogate the growth benefit of 2.5 mM glucose, as seen in (A, B). Glucose alone (B) and pyruvate alone (B) enhanced MDA-MB-231 cell growth similarly, while both together enhanced growth synergistically (B). (C) Pyruvate does not modulate glucose uptake in MDA-MB-231 cells (over a range of 1.25 mM to 5 mM). Data are displayed as mean±SD (n = 3 for A, B and n = 4 for C); * p<0.05, ** p<0.005, *** p<0.0005 by two-tailed, unpaired, unequal variance Student’s T-test (compared to Dep or as indicated by lines between subgroups).