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Time-lapse imaging of second polar body (PB) extrusion and pronuclear (PN) formation and fading of the 1PN 2PB ICSI zygote that produced an ongoing pregnancy and the delivery of a healthy child. The time of image captures (hours post ICSI) is shown at the bottom left of each image. a. First PB. b Extrusion of the second PB. c–e Formation of 1PN. f Fading of PN
