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. 2017 Jun 19;7:3811. doi: 10.1038/s41598-017-03798-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation and characterisation of nanoparticles used in this study. (A) Pfs25-IMX313 (1). Schematic of construct design particle and formation illustrating monomer (i) and heptamer (ii). (2) SDS-PAGE showing reduced monomer (i) and non-reduced heptamer (ii). (3) Size exclusion chromatography trace illustrating heptamer migration in the expected range. (B) Pfs25-SpyTag:SpyCatcher-AP205 (1) Schematic of construct design and particle formation illustrating SpyCatcher-AP205 (i), Pfs25-SpyTag (ii), and Pfs25-SpyTag:SpyCatcher-AP205 conjugate (iii). (2) Reducing SDS-PAGE showing SpyCatcher-AP205 (i), Pfs25-SpyTag (ii), and Pfs25-SpyTag:SpyCatcher-AP205 conjugate (iii). (3) Negatively stained TEM of Pfs25-SpyTag:SpyCatcher-AP205, scale bar = 50 nm and size exclusion chromatography trace illustrating VLP migration in the expected range. (C) Pfs25-Qβ (1) Schematic of construct design and particle formation illustrating Qβ VLPs (i), Pfs25 (ii), and Pfs25-Qβ conjugate (iii). (2) Reducing SDS-PAGE showing Qβ VLPs (i), Pfs25 (ii), and Pfs25-Qβ conjugate (iii). (3) Negatively stained TEM of Pfs25-Qβ VLPs, scale bar = 50 nm and size exclusion chromatography trace illustrating VLP migration in the expected range. Numbers at the left of the coomassie images represent size in kDa. Size markers in all size exclusion traces are from left to right: 670, 158, 75, 44, 29 and 13.7 kDa.