Table 2.

IC₅₀ values (efficient concentration that causes a 50% decrease in cell viability) of mastic oil (48 h and 72 h) and its constituents (72 h) against colon cancer cell lines. Data are representative of at least three independent experiments and are presented as mean ± SD (n = 6).

Cell line	Mastic oil IC50, 48 h (mg/ml)	Mastic oil IC50, 72 h (mg/ml)	α-pinene IC50, 72 h (mg/ml)	Myrcene IC50, 72 h (mg/ml)	β-pinene IC50, 72 h (mg/ml)	Limonene IC50, 72 h (mg/ml)	Linalol IC50, 72 h (mg/ml)	Combo IC50, 72 h (mg/ml)
CT26	0.1335 ± 0.0540	0.0104 ± 0.0004	0.2433 ± 0.0835	n.d.*	n.d.	0.4915 ± 0.0425	0.1540 ± 0.0267	0.0251 ± 0.0077
Caco-2	0.0368 ± 0.0225	0.0176 ± 0.0035	0.0720 ± 0.0012	0.6300 ± 0.0150	0.3700 ± 0.0701	0.0901 ± 0.0042	n.d.	0.0760 ± 0.0065
HT29	0.1751 ± 0.0028	0.0762 ± 0.0057	0.4837 ± 0.1211	n.d.	n.d.	0.6966 ± 0.0122	0.8428 ± 0.0126	0.4600 ± 0.0335

^* n.d.: not detected (not possible to determine efficient concentration that causes 50% decrease in cell viability).