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. 2017 Jun 19;7:3828. doi: 10.1038/s41598-017-03201-1

Figure 7.

Figure 7

YHC, GKW dose-dependently decrease p-ERK and downstream p-CREB protein expression level, while GRA increase their protein expression level. The mIMCD3 cells were planted in 6 well plate and cultured in RPMI1640 medium supplied with 10% fetal bovine serum. All doses were given during cell logarithmic phase for 24 hours. (A) Blotting image of p-ERK, ERK, p-CREB and CREB expressed by mIMCD3 cells treated by GRA. Bar chart of fold changes are given, and concentrations are labeled behind. The blots was cropped properly and the full length blots are presented in Supplementary Fig. S9. (B) Blotting image of p-ERK, ERK, p-CREB and CREB expressed by mIMCD3 cells treated by YHC, GKW singly or in combination with GRA, for 24 hours. When GRA was combined, the working concentration was 12 μM. The blots was cropped properly and the full length blots are presented in Supplementary Fig. S9. (C to D) Bar chart for p-ERK and p-ERK expression with different treatment. *P < 0.05 when compared with control group (none drug-treated), & P < 0.05 compared between groups with or without GRA treatment (n = 3). Total ERK and CREB level were used as internal reference respectively for calculating p-ERK and p-CREB expression. The involvement of ERK-MAPK pathway in AQP 2 expression are showed in Supplementary Fig. S8.