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. 2017 Jun 19;7:3789. doi: 10.1038/s41598-017-03922-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Workflow of HR-HDXMS. (A) Amino acid subfragments are determined from overlapping peptides generated by proteolytic digest of the protein. (B) The rate of hydrogen-deuterium exchange (k _ex) of each subfragment is calculated by linear and non-linear least squares analysis. (C) The free energy value ΔG_ex derived from k _ex is used to generate a stability fingerprint of the protein. (D) The protein fingerprint helps in understanding protein dynamics and allows predictions about residues that might be essential for protein stability and/or function.