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. 2017 Jun 19;7:3831. doi: 10.1038/s41598-017-03940-1

Figure 3.

Figure 3

Trypsin mediated BSA digestion in the presence of PBA or TUDCA: Trypsin mediated BSA digestion was analyzed by 12% SDS-PAGE (Panel A) and also by 8% Native-PAGE (Panel B) at 30° C and 75° C for 30 min. BSA (1 mg/ml) was treated with trypsin (50 µg/ml) for 10 min at 30° C in the presence and absence of 10 mM PBA or TUDCA. To determine trypsin activity on aggregated protein, BSA samples were heat treated at 75° C for 30 min in the presence and absence of 10 mM PBA or TUDCA and brought to room temperature before trypsin was added. Digested products were then analyzed by 12% SDS-PAGE and Native-PAGE. Various lanes are as follows: 1, native BSA; lanes 2–6 are native BSA treated with trypsin; trypsin + 10 mM PBA; trypsin + 10 mM TUDCA; 10 mM PBA alone and 10 mM TUDCA alone respectively; lane 7, heat treated BSA at 75° C for 30 min; 8, heat treated BSA + trypsin; 9, BSA + 10 mM PBA + heat + trypsin; 10, BSA + 10 mM TUDCA + heat + trypsin; 11 and 12, BSA + 10 mM PBA + heat and BSA + 10 mM TUDCA + heat respectively.