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. 2017 Jun 19;7:3831. doi: 10.1038/s41598-017-03940-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HepG2 cell viability in the presence of PBA or TUDCA: HepG2 cell viability was measured by MTT assay as described in ‘Materials and Methods’. Panel A: Cells were treated with different concentrations of PBA (0.5–20 mM) or with 12 µM tunicamycin for 48 h. Panel B: IC50 value for PBA as determined by using GraphPad Prism 6 software. Panel C: Cell viability in the presence of different concentrations of TUDCA (0.25–10 mM) or 12 µM tunicamycin for 48 h. Data shown are mean ± SD, (n = 3). The asterisks represent statistically significant differences (*P < 0.05, **P < 0.01). P value is calculated by t-test.