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. 2017 Jun 19;7:3800. doi: 10.1038/s41598-017-04003-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence analysis of MAT2A protein in bovine oocytes and preimplantation embryos. Confocal images that transverse at least one nucleus or pronucleus are shown. (A) Immature oocyte, (B) mature oocyte, (C) 1-cell, (D) 2-cell, (E) 8-cell, (F) morula, (G) blastocyst, (H) hatched blastocyst, and (I) negative control in which the primary antibody was omitted from the immunofluorescence of a hatched blastocyst sample. Scale bars represent 50 µm. (A’–I’) Nuclear counterstaining with propidium iodide. (J) and (K) Immunoblotting of 1-cell embryo (n = 120) and blastocyst (n = 120) lysates using the monoclonal MAT2A antibody used in the immunofluorescence and ChIP-seq analyses. Hep G2 Cell Lysate (50 µg protein) was used as a positive control.