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. 2017 Jun 19;7:3818. doi: 10.1038/s41598-017-03786-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ultrarapid Diagnosis of HIV. (A) HIV reverse transcriptase luciferase protein extract was used for LIPSTICKS antibody measurements with a cohort of HIV negative (blue) and HIV positive individuals (red). Each dot represent an individual subject and the geometric mean of the antibody levels in each group is shown by the colored horizontal bar. Based on the cut-off value (denote by the dotted line) for determining seropositivity, the performance of the test shows 100% sensitivity and 100% specificity for HIV diagnosis. P values were calculated using the Mann-Whitney U test. (B) Testing the same cohort of HIV negative (blue) and HIV positive individuals (red) with an extract of HIV p24 luciferase fusion protein demonstrated 96% sensitivity and 100% specificity. P values were calculated using the Mann-Whitney U test.