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. 2017 Jun 19;7:3759. doi: 10.1038/s41598-017-03696-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sex differences in eicosanoid biosynthesis in carrageenan-induced pleurisy in rats. Rats were treated with carrageenan (intrathoracic injection), sacrificed at the given time points, and (A) LTB₄ and (B) PGE₂ in the thoracic exudates were measured by EIA and RIA, respectively. (C) 6-keto-PGF_1α levels in thoracic exudates were measured 4 hrs after carrageenan injection by EIA. Time zero (T0) corresponds to untreated rats. Values represent means ± S.E.M; n = 6 rats/T0; n = 13 rats/2 hrs; n = 6 rats/4 hrs; n = 6 rats/8 hrs; ^#p < 0.05; ^###p < 0.001, male vs female; two-way ANOVA plus Bonferroni (A and B) and two-tailed Student’s t test (C).