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. 2017 Jun 19;7:3838. doi: 10.1038/s41598-017-03878-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic of method for transcriptome analysis of developing maize endosperm. (A–C) HistoGene-stained frozen sections from kernels at (A) 8 DAP, (B) 12 DAP, and (C) 16 DAP. Three insets on the right side of each panel show the three tissue types isolated from cryo-sections by free-hand dissection. The tissues surrounded by the red dashed lines and the blue dashed lines correspond to the AL and SE tissue, respectively. The BETL is marked with green dashed lines. NC, nucellus. (D) The bioinformatics pipeline used to identify spatio-temporally regulated coding and long noncoding transcripts at three stages of the maize endosperm development.