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. 2017 Jun 19;7:3758. doi: 10.1038/s41598-017-03869-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Modeling chronic β cell lipotoxicity to mimic in vivo effects of chronic HFD. MIN-6 β cells were treated with 40 hours of palmitate + a cytokine mix to mimic chronic lipotoxicity and inflammation (n = 4). (a) Representative global PRP tracing. (b) Puromycin incorporation of cells from both treatment groups, with quantification of total band intensities. (c–e) PRP analysis of individual transcripts for Ins1/2, Ddit3, and Atf4 by fraction.(f–h) Quantification of P/M Ratios for each transcript. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.