Table 7.
Effect of isoflavonoids on the accumulation of ethidium bromide by fluorometry.
| SA1199B | RWW337 | M116 | RWW50 | |
|---|---|---|---|---|
| Reserpine | 0.53 ± 0.19 | 0.25 ± 0.10 | 0.62 ± 0.14 | 0.75 ± 0.21 |
| Daidzein | 0.33 ± 0.10 | 0.05 ± 0.02 | 0.05 ± 0.09 | −0.08 ± 0.06 |
| Genistein | 0.67 ± 0.19 | 0.20 ± 0.10 | 0.13 ± 0.11 | 0.12 ± 0.07 |
| Tectorigenin | 0.81 ± 0.12 | 0.19 ± 0.09 | 0.42 ± 0.07 | 0.47 ± 0.04 |
| Glycitein | 0.31 ± 0.10 | 0.06 ± 0.05 | −0.02 ± 0.00 | 0.07 ± 0.05 |
| Biochanin A | 0.44 ± 0.05 | 0.63 ± 0.15 | 0.33 ± 0.08 | 0.47 ± 0.08 |
| Calycosin | 0.63 ± 0.11 | 0.19 ± 0.13 | 0.22 ± 0.03 | 0.34 ± 0.13 |
| Irisflorentin | 0.23 ± 0.03 | 0.05 ± 0.11 | 0.30 ± 0.05 | 0.02 ± 0.08 |
| Irigenin | 0.48 ± 0.15 | 0.15 ± 0.10 | 0.28 ± 0.04 | 0.34 ± 0.06 |
| Orobol | 0.76 ± 0.18 | 0.07 ± 0.04 | 0.08 ± 0.14 | −0.13 ± 0.01 |
| Apigenin | 0.22 ± 0.05 | 0.01 ± 0.05 | 0.04 ± 0.03 | 0.05 ± 0.05 |
| Chrysin | 0.09 ± 0.07 | −0.03 ± 0.02 | −0.03 ± 0.10 | 0.01 ± 0.01 |
Relative fluorescence (RF) values were calculated for all isoflavonoids by fluorometric method in SA1199B and three MRSA strains. Reserpine was used as positive efflux pump inhibitor control. The flavonoids apigenin and chrysin found in Cytisus striatus leaves were included in the assay. The RF values are the averages and standard deviations of at least three independent assays.