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. 2017 Jun 19;7:3780. doi: 10.1038/s41598-017-03245-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Behaviour of Scyphax ornatus in six test experiments (rows 1–6). (1) Constant darkness, n = 20; (2) T = 24 hours, n = 25; (3) T = 23 hours, n = 20; (4) T = 24.3 hours, n = 18; (5) Tidal T = 12.25 hours, n = 19; (6) Tidal T = 12.65 hours, n = 20. Column (A) shows double plotted activity plots (actograms). The actograms represent the combined total of the normalized individuals (see Supplementary Information S2 for all individual actograms). Black bars indicate activity. The lights and tide signals are shown in yellow and blue, respectively, on the left-hand panel only. Column (B) indicates the model fit with the beats-predicted period. The black line indicates the observed amount of Scyphax activity in the analysis window per night. The red line shows the model fit. Grey bars represent nights when the tidal signal coincided with the analysis window; heights of bars indicate the duration of coincidence. (See also S1 for periodogram analysis of the shorter period circatidal and circadian rhythms in the data).