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. 2017 Jun 19;7:3764. doi: 10.1038/s41598-017-03821-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetically distinct from other Mollicutes, Ca. M. girerdii strains are distinguished from each other by a small set of variable features. Displayed are the highest identity database hits for Ca. M. girerdii strain UC-B3 proteins. Sequences (n = 574) were queried against the UniRef100 database (downloaded December 2015) using USEARCH in local alignment mode with the settings described in the Supplementary Methods. Under these search conditions, 8 sequences had no hit (not plotted), 176 had only 1 hit (plotted as ‘best’), and 390 had at least 2 hits (plotted as ‘best’ and ‘2^nd best’ by identity). Also indicated is whether or not the hit was from closely related strain VCU-M1. USEARCH is most effective at protein identities ≥50% (dotted line). On the x-axis, sequences are numbered in order of appearance in the UC-B3 genome.