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. 2017 Jun 19;7:3805. doi: 10.1038/s41598-017-04008-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The apoptosis rate and caspase-3 levels of rats’ hypothalamic neuroendocrine cells at 12 h and 24 h. (A) Shows the apoptosis rate of rat hypothalamic neuroendocrine cells after modeling for 12 h and 24 h. (B) Shows the Caspase-3 levels of rat hypothalamic neuroendocrine cells after modeling for 12 h and 24 h (Western blot). (C) Shows AOD of Caspase-3. (D) Shows the tissue lysates for protein extraction were prepared and the total protein of each lysate was equalized for Western blot analysis. Proteins were detected by specific antibodies to Caspase-3 and PCNA. β-actin was used as a loading control. (E) Shows immunostaining of Caspase-3 (×400). *P < 0.05 vs Normal 12 h (24 h), ^# P < 0.05 vs Sham 12 h (24 h).