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. 2017 Jun 19;7:3805. doi: 10.1038/s41598-017-04008-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The immunohistochemistry images show apoptosis of rabbit hypothalamic and hypophyseal neuroendocrine cells at 12 h and 24 h (×400). (A–F) Show the apoptosis of rabbit hypothalamic neuroendocrine cells at 12 h and 24 h. (G–L) Show the apoptosis of rabbit hypophyseal neuroendocrine cells at 12 h and 24 h. (A,G) Show Normal 12 h. (B,H) Show Restraint 12 h. (C,I) Show R + E group 12 h. (D,J) Show Normal 24 h. (E,K) Show Restraint 24 h. (F,L) Show R + E 24 h. The arrows in the panels show the apoptotic cells.