Figure 3.

Relative quantitation compared to control diet of 16S rRNA copy numbers of rumen microbiota groups after supplementation of the control diet with sunflower oil (SFL) or with combinations of SFL and fish oil (FSH) or marine algae (ALG). Fold-changes for specific amplicon groups were calculated as the (log 2) ratio of normalized abundances. (A) Total bacteria and microbial groups involved in biohydrogenation. (B) Major prokaryotic and eukaryotic domains found in the rumen including the Archaeal groups responsible for the production of ruminal methane. (C) Other key bacteria groups including the dominant Prevotella spp. and species involved in fiber degradation, and lactate metabolism. ^*Significant difference in copy number (P < 0.05) compared with the control.