Table 1.
Quantitative real-time PCR primers used in this study.
| Gene | Forward primer (5′3′) | Reverse primer (5′3′) | Product size (bp) | GenBank accession no. |
|---|---|---|---|---|
| GAPDH | CCTCTCTGGCAAAGTCCAAG | CATCTGCCCATTTGATGTTG | 200 | NM_204305 |
| TLR3 | ACAATGGCAGATTGTAGTCACCT | GCACAATCCTGGTTTCAGTTTAG | 123 | NM_001011691 |
| TLR7 | TGTGATGTGGAAGCCTTTGA | ATTATCTTTGGGCCCCAGTC | 218 | DQ780342 |
| S1PR1 | TTGCCTTGTCAGCTTCTGTG | CGTGGAGCAGTTTGACAAGA | 203 | XM_422305 |
| IL-1β | GCTCTACATGTCGTGTGTGATGAG | TGTCGATGTCCCGCATGA | 80 | NM_204524 |
| IL-6 | CCTGTTCGCCTTTCAGACCT | GGGATGACCACTTCATCGGG | 171 | EU170468 |
| IL-8 | ATTCAAGATGTGAAGCTGAC | AGGATCTGCAATTAACATGAGG | 196 | DQ393272 |
| TNF-α | CCGCCCAGTTCAGATGAGTT | GCAACAACCAGCTATGCACC | 130 | AY765397 |
| IFN-α | ATGCCACCTTCTCTCACGAC | AGGCGCTGTAATCGTTGTCT | 387 | EU367971 |
| IFN-β | CCTCAACCAGATCCAGCATT | GGATGAGGCTGTGAGAGGAG | 259 | AY831397 |
| IFN-γ | TGAGCCAGATTGTTTCGATG | CTTGGCCAGGTCCATGATA | 248 | DQ906156 |
| CCL5 | GTTTGGGGCTGATACAACCG | CCTTCACATGATTCTGGGGCA | 71 | NM_001045832 |
| MHC-I | AAGAAGGGGAAGGGCTACAA | AAGCAGTGCAGGCAAAGAAT | 222 | NM_001031338 |
| MHC-II | CTCGAGGTCATGATCAGCAA | TGTAAACGTCTCCCCTTTGG | 312 | DQ008588 |