Table 1.

Cell Counting Kit-8 assay results and IC₅₀ for 21 batches of Polygonum multiflorum (means ± SD).

Batch NO.	Concentration (mg crude drug/mL)						IC50
	2	1	0.5	0.25	0.125	0.0625
1	14.34 ± 0.06	20.46 ± 0.49	48.84 ± 2.16	79.89 ± 2.92	95.67 ± 0.88	99.1 ± 1.18	0.83
2	13.05 ± 0.42	15.21 ± 0.59	40.62 ± 0.67	79.63 ± 5.70	88.32 ± 2.15	95.15 ± 2.65	0.757
3	14.33 ± 2.42	14.64 ± 0.31	32.01 ± 1.56	73.14 ± 1.65	95.44 ± 3.34	99.81 ± 9.37	0.715
4	13.32 ± 0.92	17.62 ± 0.15	32.48 ± 1.95	60.4 ± 1.58	84.14 ± 1.14	98.53 ± 7.74	0.687
5	14.5 ± 0.33	16.1 ± 0.61	44.61 ± 2.04	73.85 ± 3.61	92.04 ± 6.45	95.35 ± 6.52	0.779
6	11.4 ± 2.08	15.08 ± 2.66	39.76 ± 2.14	69.53 ± 2.52	86.1 ± 3.56	94.36 ± 4.92	0.692
7	7.11 ± 0.42	41.2 ± 0.49	72.7 ± 2.28	86.2 ± 2.77	90.81 ± 2.34	93.58 ± 1.14	0.944
8	14.72 ± 0.32	54.74 ± 1.12	78.76 ± 4.42	86.69 ± 1.43	92.37 ± 2.86	93.15 ± 1.63	1.151
9	41.81 ± 1.25	74.14 ± 2.62	85.8 ± 2.74	88.12 ± 0.66	96.55 ± 6.47	98.87 ± 4.50	1.73
10	8.8 ± 0.60	59.68 ± 0.91	89.83 ± 0.43	91.27 ± 3.22	96.19 ± 7.60	98.45 ± 0.31	1.177
11	18.15 ± 3.69	70.09 ± 4.47	90.46 ± 3.62	97.28 ± 10.64	102.04 ± 7.52	102.41 ± 9.82	1.383
12	36.44 ± 1.56	71.8 ± 7.75	87.57 ± 8.66	93.19 ± 12.92	98.18 ± 7.86	101.24 ± 1.40	1.629
13	34.75 ± 2.43	75.57 ± 2.31	94.37 ± 1.55	97.56 ± 2.96	97.32 ± 1.85	101.17 ± 2.85	1.656
14	17.17 ± 2.24	51.62 ± 1.88	80.41 ± 3.36	90.95 ± 5.74	94.75 ± 4.52	98.98 ± 4.76	1.199
15	34.62 ± 4.39	70.94 ± 10.66	87.8 ± 5.36	93.14 ± 6.65	96.17 ± 11.97	100.06 ± 4.16	1.595
16	32.64 ± 3.44	67.14 ± 2.48	85.43 ± 6.43	93.94 ± 7.34	95.51 ± 13.55	98.95 ± 8.65	1.527
17	16.65 ± 0.52	51.36 ± 3.03	86.82 ± 2.35	92.07 ± 3.44	95.24 ± 6.59	96.27 ± 3.72	1.213
18	17.27 ± 2.33	54.25 ± 2.58	83.96 ± 1.94	88.16 ± 6.78	88.92 ± 1.83	90.44 ± 1.16	1.195
19	26.83 ± 1.45	72.48 ± 3.09	93.47 ± 5.56	94.69 ± 3.25	101.31 ± 4.30	101.2 ± 12.36	1.515
20	30.74 ± 1.42	67.48 ± 7.03	87.54 ± 3.40	93.71 ± 2.37	96.61 ± 3.79	100.36 ± 8.12	1.382
21	31.3 ± 2.39	71.67 ± 3.19	88.91 ± 5.47	98 ± 3.84	99.35 ± 2.90	99.95 ± 1.29	1.562