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. 2017 Jun 20;7:260. doi: 10.3389/fcimb.2017.00260

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Copyright © 2017 Hart, Abraham and Aly.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The morphology of Pyppcs(−) and Pyppcdc(−) oocysts appear diminished in size. Fluorescence and bright field microscopy images of ookinetes 20 hours pmf (400X magnification), oocysts at day 4 pmf (100X magnification), oocysts at day 7 pmf (100X magnification), and oocysts at day 7 pmf (400X magnification) dissected out of mosquitoes infected with Pyppcs(−), Pyppcdc(−), or Pyp230p(−) strains. Images show the morphology of Pyppcs(−) and Pyppcdc(-) oocysts appear diminished in size and fluorescence compared to the oocysts of the oocysts of Pyp230p(−), but the ookinetes of all three strains appear normal in size and morphology.