Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 20;7:269. doi: 10.3389/fcimb.2017.00269

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Ma, Ye, Chen, Nie, Cheng, Zhang, Han, Wu, Xu, Lei and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Performance comparison of the ICW assay with conventional assays. HUVECs and A549 cells grown in 6-well plate until they reached 60–70% confluence were mock infected or infected with HTNV at an MOI of 0.1 and then harvested at 0 dpi, 1 dpi, 2 dpi, 3 dpi, and 4 dpi for qRT-PCR to detect the HTNV S segment. These samples were used to calculate the viral loads (A) or for Western blotting to measure HTNV NP expression (B). HUVECs infected at an MOI of 0.1 also underwent flow cytometry analysis (C,D) or an immunofluorescence assay (E,F) at the indicated time points post-HTNV infection. Data are presented as the mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. 0 dpi by Student's t-test. The experiments were performed independently at least three times with similar results.