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Expression profiling of FLQ and TLQ in cytoplasm and nuclear fractions at different time points. N2a cells were transfected with FLQ or TLQ constructs, and collected for Western blotting using cytoplasm and nuclear fractions 24 (A), 36 (B), and 48 (C) h after transfection. The expression of exogenous ATXN3 is indicated by arrow heads. α-tubulin was used as a marker for the cytoplasm fraction, and histone H3 was used as a marker for the nuclear fraction.
