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. 2016 Oct 11;7:13103. doi: 10.1038/ncomms13103

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Copyright © 2016, The Author(s)

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(a,b) NIH/3T3 cells were transfected with 4 μg of Flag-tagged WT and catalytically inactive version of NRK1 or NRK2 overexpressing vector. Nmrk1 and Nmrk2 mRNA (a) and NRK1/NRK2 protein (b) expression. (c) NAD⁺ measurement in NR-treated NIH/3T3 cells overexpressing NRK1 or NRK2. Results shown are mean±s.e.m.; EV, empty vector; *P<0.05 versus ctrl by two-sided unpaired T-test (n=3).