Skip to main content
. 2016 Oct 11;7:13103. doi: 10.1038/ncomms13103

Figure 5. NRK1 is essential for NR and NMN to enhance NAD+ synthesis in hepatic cells.

Figure 5

(a,b) AML12 cells were transfected with NRK1 siRNA. (a) Protein and mRNA levels of NRK1 and other NAD+-related enzymes. (b) NAD+ measurement after 6 h 0.5 mM NR treatment. *P<0.05 versus ctrl by two-sided unpaired T-test. (c–e) Primary hepatocytes were isolated from 10 to 15 weeks old WT and NRK1KO male mice. Protein (c) and mRNA (d) levels of NRK1 and other enzymes involved in NAD+ metabolism. (e) NAD+ measurement after 6 h treatment with 0.5 mM NR, 0.5 mM NMN, 5 mM NAM, 0.5 mM NA or vehicle control. Results shown are mean±s.e.m., *P<0.05 versus WT by two-sided unpaired T-test. (n=3).