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. 2016 Oct 11;7:13103. doi: 10.1038/ncomms13103

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Five to six weeks old WT and NRK1KO male mice were IP injected with 500 mg kg⁻¹ of the indicated compounds. Then, liver, kidney, brown adipose (BAT) and skeletal muscle samples were collected after 1 h, n=5 per group. NAD⁺ measurement in liver (a), total kidney extracts (c), BAT (e) and muscle (g) tissue. Percentage increase above basal NAD⁺ level in liver (b), kidney (d), BAT (f) and muscle (h) tissue upon treatments. Results shown are mean±s.e.m., *P<0.05, **P<0.01, ***P<0.001 versus WT by two-sided unpaired T-test (n=5).