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. 2017 Jun 6;2017:5958429. doi: 10.1155/2017/5958429

Figure 2.

Figure 2

STAT3 and NF-κB bind upon IL-6 and TNF-α treatments in colorectal cancer cell lines DLD and HT-29. (a) Immunoprecipitation with STAT3 antibody revealed that NF-κB was bound to STAT3 in DLD1 cell line. STAT3 pull-down was performed in the presence of IL-6 (10 ng/ml) and TNF-α (25 ng/ml). (b) In the presence of withaferin A (10 μM), pull-down assay with STAT3 antibody showed that STAT3 interaction with NF-κB was abolished in DLD1 cell line. (c) Immunoprecipitation with STAT3 antibody showed the interactions with NF-κB in HT-29 cell line. (d) With the withaferin A challenge (10 μM), STAT3 and NF-κB interactions were abolished in HT-29.