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. 2017 Jun 6;2017:5958429. doi: 10.1155/2017/5958429

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Copyright © 2017 Seyung S. Chung et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

STAT3 dimerize with STAT1 and STAT1 dimerize with NF-κB. Withaferin A abolished the protein-protein interactions. (a) STAT1 was heterodimerized with STAT3 from the pull-down assay with an STAT3 antibody. The binding affinity was increased upon IL-6 and TNF-α cotreatments. (b) Withaferin A (10 μM) treatment has abolished the STAT3-STAT1 interactions. (c) Coimmunoprecipitation with STAT1 antibody revealed that STAT1 was bound to NF-κB. STAT1-NF-κB interaction was enhanced by the IL-6 and TNF-α stimulation. (d) These interactions were also abolished by withaferin A (10 μM) treatment.