Table 1.
PCR primers and single base pair (bp) extension primers of 4 SNPs at IL-1β, GDF5, MMP-3, and TGF-β1.
| Genes | SNPs | PCR primer sequence (5′→3′) | Single bp extension primer sequence |
|---|---|---|---|
| IL-1β | rs1143627-F | ACGTTGGATGTTGTGCCTCGAAGAGGTTTG | TCCCTCGCTGTTTTTAT |
| rs1143627-R | ACGTTGGATGTCTCAGCCTCCTACTTCTGC | ||
| MMP-3 | rs650108-F | ACGTTGGATGTCAGGTAGAGGTGACAAGTG | CCAGTGGGTGAGGTTAGA |
| rs650108-R | ACGTTGGATGGTCACTGTCTCATTGTGTGT | ||
| TGF-β1 | rs1800469-F | ACGTTGGATGTACAGGTGTCTGCCTCCTGA | ACTCCTGACCCTTCCATCC |
| rs1800469-R | ACGTTGGATGAAGAGGGTCTGTCAACATGG | ||
| GDF5 | rs143383-F | ACGTTGGATGCAGCAGCTGAAAATAACTCG | TCTTGAAAGGAGAAAGCC |
| rs143383-R | ACGTTGGATGTTCAAGCCCTCAGTCAGTTG |
PCR: polymerase chain reaction; SNP: single nucleotide polymorphism.