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. 2017 Jun 20;8:140. doi: 10.3389/fendo.2017.00140

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Copyright © 2017 Mohideen-Abdul, Tazibt, Bourguet, Hazemann, Lebars, Takacs, Cianférani, Klaholz, Moras and Billas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Titrations of DNAs by ERRα DNA-binding domain (DBD). Imino protons region of 1D spectra recorded at 20°C in 50 mM sodium phosphate buffer (pH 7.0), 5 mM MgCl₂, and 1 mM TCEP in 90/10 H₂O/D₂O. The sequences and secondary structures of the DNAs are indicated. The assignments of imino proton based on NOESY experiments are indicated for free DNAs (top). The ratios (ERRα DBD:DNA) are indicated on the left. Broken lines indicate imino protons that undergo chemical shift changes (red in the sequence) and lines indicate imino proton that remain unchanged (blue in the sequence). (A) Titration of tff1 ERE/IR3 (26) DNA. Tentative assignments are indicated in italics. (B) Titration of lf estrogen-related response element (ERRE) (26) DNA. (C) Titration of tra ERRE (26) DNA. (D) Titration of rb1cc1 IR3 (26) DNA. (E) Titration of embERRE/IR3 (26) DNA.