Fig. 1. Design, structure and heme binding of four stranded β-sheet peptide-1. (A) Model helical peptide with a heme (engineered glycophorin A) and a β-barrel heme protein (PDB ID: 3CSN). (B) Design of peptide-1 with residues involved in heme ligation (red). (C) Section of a two-dimensional 1H–1H NOESY spectrum showing NOE connectivity between amide protons. Long range NOEs are underlined and boldfaced. (D) Superimposed twenty lowest energy structures of peptide-1. (E) Ribbon representation of the peptide-1 structure with side chains. (F) Heme binding isotherm of peptide-1.
