Fig. 3. Design, structure and heme binding of six stranded β-sheet peptides. (A) Design of peptide-8 with His residues involved in heme ligation are shown in red. (B) Heme binding isotherms of peptide-8 (red), peptide-8H6AH12A or 9 (blue) and peptide-8H15AH21A or 10 (pink). (C) Superimposed ten lowest energy structures of peptide-8. (D) Model structure of the heme–peptide-8 complex. The side chains of the four axial ligands coordinating heme are shown (red). Residues involved in side chain packing within β-sheets are highlighted (gold).
