Table 1. Summary of heme binding affinity and enzymatic efficacy of the designed peptides a .
Peptide | K d μM | k cat/Km × 107 M–1 s–1 |
1 | 5.8 ± 0.7 | 2.84 |
2 | 8.9 ± 0.9 | 2.91 |
3 | 2.7 ± 0.5 | 2.79 |
4 | 2.0 ± 0.2 | 2.44 |
5 | 1.6 ± 0.2 | 1.57 |
6 | 0.8 ± 0.2 | 1.34 |
7 | 0.4 ± 0.2 | 1.15 |
8 | 0.5 ± 0.1 | 4.35 |
9 | 28.1 ± 3.2 | 2.21 |
10 | 19.5 ± 2.1 | 1.30 |
a9 and 10 represent peptide-8H6AH12A and peptide-8H15AH21A, respectively.