Fig. 3. Calibration plots for the quantification of A) PSA, B) AFP, and C) CEA. Different concentration of antigens were incubated with the Ab1-MNP and the Ab2, error bar, standard error of mean, n = 3. D) Calibration plot for the quantification of PSA in the presence of 10 % glycerol (top) and in the absence of 10 % glycerol (bottom), error bar, standard error of mean, n = 3. (Average net intensity = (1×1 sq pixel of 50 individual MICs) – (1×1 sq pixel of 50 individual background area on the image)/50).
