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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Mucosal Immunol. 2017 Mar 1;10(4):1069–1081. doi: 10.1038/mi.2017.15

Figure 1. Infection with Mtb HN878 triggers increased IL-22 production through a TLR2-dependent pathway.

Figure 1

Lung cells isolated from B6, Il1r-/- and Tlr2-/- mice were infected with either Mtb H37Rv (Rv) or Mtb HN878 (HN) at MOI of 0.1 for 7 days, and the protein levels of (a) IL-1β and (b) IL-22 were assessed in culture supernatants by ELISA and luminex assay, respectively. Lung cells isolated from B6 mice were treated with cell wall extracted from Mtb H37Rv (Rv) or Mtb HN878 (HN), (10μg/ml each). Protein levels of (c) IL-1β and (d) IL-22 were assessed by ELISA and luminex assay respectively. PBMCs were isolated from ATB patients and were stimulated with Mtb H37Rv (Rv) or Mtb HN878 (HN) cell wall extracts (20 μg/ml each) and the protein levels of (e) IL-22 were measured by luminex assays. Error bars represent means ± s.e.m. n = 3-5 for a-d. n = 17 for human ATB patients for e. ND, not detected; UI, uninfected; UN, untreated. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 by (a,b) one-way ANOVA, (c,d) Student's t test (e) two-way ANOVA