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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Mucosal Immunol. 2017 Mar 1;10(4):1069–1081. doi: 10.1038/mi.2017.15

Figure 6. IL-22 is required for macrophage and monocyte recruitment during the chronic stages of Mtb HN878 infection.

Figure 6

Il22-/- and B6 mice were aerosol infected with ∼500 CFU of Mtb HN878 and lungs harvested at different time points p.i. The total number of (a) alveolar macrophages (AMs), (b) monocytes (Mn), (c) recruited macrophages (RM), (d) neutrophils (Neu) were determined by flow cytometry. (e) FFPE lung sections were processed for lung pathology and immunofluorescence using antibodies specific for F4/80 (red) and IL-22R (green). All sections were counterstained with DAPI (blue). Circles indicate region of interest for F4/80+ and IL-22R+ within the granulomas. (f) Quantification of IL22R+ cells in lung sections from (e) were counted /200× field. Error bars represent standard deviations (± s.d.). n = 5 mice, Representative individual experiments are shown from at least one experiment. **P < 0.01, ***P < 0.001 by Student's t test.