Figure 2.

Efficient Vector Transduction Exhibited in Transverse Retinal Sections

(A–H) GFP expression was detectable throughout the retinal layers (A–D) (original magnification ×2 in E–H) from retinal ganglion cells to CD31-positive endothelial cells present in areas where choroidal neovascularization (CNV) was induced via laser photocoagulation (arrows in E and F), demonstrating the efficient transduction of rAAV-mTOR shRNA into cells of the outermost retinal layer. Scale bars, 100 μm. ONL, outer nuclear layer; RPE, retinal pigment epithelium.